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Hollfelder Group


 For the very latest publications check on PubMed through this link (opens a new window).


2023 2022 2021 2020 / 2019 / 2018 / 2017 / 2016 / 2015 / 2014 / 2013/ 2012 / 2011 / 2010 / 2009 / 2008 / 2007 / 2006 / Before 2005


  • Scheele, R.; Weber, Y.; Nintzel, F.; Herger, M.; Kaminski, T. S.; Hollfelder, F. Ultrahigh throughput evolution of tryptophan synthase in droplets via an aptamer-biosensor.. bioRxiv 2023
  • Zinchenko, A.; Devenish, S. R. A.; Hollfelder, F. Rapid quantitative assessment of small molecule leakage from microdroplets by flow cytometry and improvement of fluorophore retention in biochemical assays. bioRxiv 2023
  • Fischer, K.; Lulla, A.; So, T. Y.; Pereyra-Gerber, P.; Raybould, M. I. J.; Kohler, T. N.; Kaminski, T. S.; Yam-Puc, J. C.; Hughes, R.; Leiss-Maier, F.; Brear, P.; Matheson, N. J.; Deane, C. M.; Hyvönen, M.; Thaventhiran, J. E. D.; Hollfelder, F. Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies. bioRxiv 2023
  • Collins, S.; van Vliet, L. D.; Gielen, F.; Kanecek, M.; Wagner-Valladolid, S.; Poudel, C.; Fusco, G.; de Simone, A.; Michel, C.; Kaminski, C. F.; Spring, D. R.; Hollfelder, F.; Kaminski Schierle, G. A unified in vitro to in vivo fluorescence lifetime screening platform yields amyloid β aggregation inhibitors. bioRxiv 2022.


188. Bhattacharjee, S.; Guo, C.; Lam, E.; Holstein, J. M.; Rangel Pereira, M.; Pichler, C. M.; Pornrungroj, C.; Rahaman, M.; Uekert, T.; Hollfelder, F.; Reisner, E. Chemoenzymatic Photoreforming: A Sustainable Approach for Solar Fuel Generation from Plastic Feedstocks. J Am Chem Soc 2023, 145, 20355-20364. Read online.

187. Wilson, L. F. L.; Neun, S.; Yu, L.; Tryfona, T.; Stott, K.; Hollfelder, F.; Dupree, P. The biosynthesis, degradation, and function of cell wall beta-xylosylated xyloglucan mirrors that of arabinoxyloglucan. New Phytol 2023. Read online.

186. Schnettler, J. D.; Wang, M. S.; Gantz, M.; Karas, C.; Hollfelder. F. Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library of De Novo Designed Proteins, 2023, submitted (preprint).

185. Ladeveze, S.; Zurek, P.; Kaminski, T. S.; Emond, S.; Hollfelder, F. Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets. ACS Catalysis 2023, 13, 10232-10243. available online

184. de Jonghe, J.; Kaminski, T. S.; Morse, D. B.; Tabaka, M.; Ellermann, A. L.; Kohler, T. N.; Amadei, G.; Handford, C. E.; Findlay, G. M.; Zernicka-Goetz, M.; Teichmann, S.; Hollfelder, F. spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content. Nature Communication 2023, 14, 4788. Read online

183. Kohler, T. N.; De Jonghe, J.; Ellermann, A. L.; Yanagida, A.; Herger, M.; Slatery, E. M.; Weberling, A.; Munger, C.; Fischer, K.; Mulas, C.; Winkel, A.; Ross, C.; Bergmann, S.; Franze, K.; Chalut, K.; Nichols, J.; Boroviak, T. E.; Hollfelder, F. Plakoglobin is a mechanoresponsive regulator of naive pluripotency. Nat Commun 2023, 14, 4022. Read online

182. Gantz, M.; Neun, S.; Medcalf, E. J.; van Vliet, L. D.; Hollfelder, F. Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments. Chem Rev 2023, 123, 5571-5611. Read online

181. Yam-Puc, J. C.; Hosseini, Z.; Horner, E. C.; Gerber, P. P.; Beristain-Covarrubias, N.; Hughes, R.; Lulla, A.; Rust, M.; Boston, R.; Ali, M.; Fischer, K.; Simmons-Rosello, E.; O'Reilly, M.; Robson, H.; Booth, L. H.; Kahanawita, L.; Correa-Noguera, A.; Favara, D.; Ceron-Gutierrez, L.; Keller, B.; Craxton, A.; Anderson, G. S. F.; Sun, X. M.; Elmer, A.; Saunders, C.; Bermperi, A.; Jose, S.; Kingston, N.; Mulroney, T. E.; Pinon, L. P. G.; Collaboration, C.-N. C.-B.; Chapman, M. A.; Grigoriadou, S.; MacFarlane, M.; Willis, A. E.; Patil, K. R.; Spencer, S.; Staples, E.; Warnatz, K.; Buckland, M. S.; Hollfelder, F.; Hyvonen, M.; Doffinger, R.; Parkinson, C.; Lear, S.; Matheson, N. J.; Thaventhiran, J. E. D. Age-associated B cells predict impaired humoral immunity after COVID-19 vaccination in patients receiving immune checkpoint blockade. Nat Commun 2023, 14, 3292. Read online

180. Medcalf, E. J.; Gantz, M.; Kaminski, T. S.; Hollfelder, F. Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies. Anal Chem 2023, 95, 4597-4604. Read online

179.  Dowbaj, A. M.; Kohler, T. N.; Cordero-Espinoza, L.; Hollfelder, F.; Huch, M. Generation of liver mesenchyme and ductal cell organoid co-culture using cell self-aggregation and droplet microfluidics. STAR Protoc 2023, 4, 102333. Read online

178. Knyphausen, P.; Rangel Pereira, M.; Brear, P.; Hyvonen, M.; Jermutus, L.; Hollfelder, F. Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture. Nature Communications 2023, 14(1):768. doi: 10.1038/s41467-023-36099-7t. Read online

177. Schnettler, J. D.; Klein, O. J.; Kaminski, T. S.; Colin, P. Y.; Hollfelder, F. Ultrahigh-Throughput Directed Evolution of a Metal-Free alpha/beta-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase. J Am Chem Soc 2023, 145, 1083-1096. Read online


2022(back to top)

176. Amadei, G.; Handford, C. E.; Qiu, C.; De Jonghe, J.; Greenfeld, H.; Tran, M.; Martin, B. K.; Chen, D. Y.; Aguilera-Castrejon, A.; Hanna, J. H.; Elowitz, M. B.; Hollfelder, F.; Shendure, J.; Glover, D. M.; Zernicka-Goetz, M. Embryo model completes gastrulation to neurulation and organogenesis. Nature 2022, 610, 143-153. Read online

175. Bao, M.; Cornwall-Scoones, J.; Sanchez-Vasquez, E.; Chen, D. Y.; De Jonghe, J.; Shadkhoo, S.; Hollfelder, F.; Thomson, M.; Glover, D. M.; Zernicka-Goetz, M. Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension. Nat Cell Biol 2022, 24, 1341-1349. Read online

174. Richter, E. S.; Link, A.; McGrath, J. S.; Sparrow, R. W.; Gantz, M.; Medcalf, E. J.; Hollfelder, F.; Franke, T. Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance. Lab Chip 2022, 23, 195-202. Read online

173. Neun, S.; van Vliet, L.; Hollfelder, F.; Gielen, F. High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform. Anal Chem 2022, 94, 16701-16710. Read online

172. Eenink, B. D. G.; Kaminski, T. S.; Bornberg-Bauer, E.; Jose, J.; Hollfelder, F.; van Loo, B. Vector redesign and in-droplet cell-growth improves enrichment and recovery in live Escherichia coli. Microb Biotechnol 202215, 2845-2853. Read online

171. Scheele, R. A.; Lindenburg, L. H.; Petek, M.; Schober, M.; Dalby, K. N.; Hollfelder, F. Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates. Nat Commun 2022, 13, 844. Read online

170. Salmen, F.; De Jonghe, J.; Kaminski, T. S.; Alemany, A.; Parada, G. E.; Verity-Legg, J.; Yanagida, A.; Kohler, T. N.; Battich, N.; van den Brekel, F.; Ellermann, A. L.; Arias, A. M.; Nichols, J.; Hemberg, M.; Hollfelder, F. *; van Oudenaarden, A.* High-throughput total RNA sequencing in single cells using VASA-seq. Nat Biotechnol 202240,1780-1793. Read online

169. Neun, S.; Brear, P.; Campbell, E.; Tryfona, T.; El Omari, K.; Wagner, A.; Dupree, P.; Hyvonen, M.; Hollfelder, F. Functional metagenomic screening identifies an unexpected beta-glucuronidase. Nat Chem Biol 2022, 18, 1096-1103. Read online

168. Munger, C.; Kohler, T. N.; Slatery, E.; Ellermann, A. L.; Bergmann, S.; Penfold, C.; Ampartzidis, I.; Chen, Y.; Hollfelder, F. *; Boroviak, T. E. * Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation. Development 2022, 149, 20. Read online

167. Jackson, C.; Toth-Petroczy, A.; Kolodny, R.; Hollfelder, F.; Fuxreiter, M.; Kamerlin, S. C. L.; Tokuriki, N. Adventures on the Routes of Protein Evolution-In Memoriam Dan Salah Tawfik (1955-2021). J Mol Biol 2022, 434, 167462. Read online

166. Gantz, M.; Aleku, G. A.; Hollfelder, F. Ultrahigh-throughput screening in microfluidic droplets: a faster route to new enzymes. Trends Biochem Sci 202247, 451-452. Read online.

165. Bleuez, C.; Koch, W. F.; Urbach, C.; Hollfelder, F.; Jermutus, L. Exploiting protease activation for therapy. Drug Discov Today 2022, 27, 1743-1754. Read online

164. Bergmann, S.; Penfold, C. A.; Slatery, E.; Siriwardena, D.; Drummer, C.; Clark, S.; Strawbridge, S. E.; Kishimoto, K.; Vickers, A.; Tewary, M.; Kohler, T. N.; Hollfelder, F.; Reik, W.; Sasaki, E.; Behr, R.; Boroviak, T. E. Spatial profiling of early primate gastrulation in utero. Nature 2022, 609, 136-143. Read online

163. Aleku, G. A.; Titchiner, G. R.; Roberts, G. W.; Derrington, S. R.; Marshall, J. R.; Hollfelder, F.; Turner, N. J.; Leys, D. Enzymatic N-Allylation of Primary and Secondary Amines Using Renewable Cinnamic Acids Enabled by Bacterial Reductive Aminases. ACS Sustain Chem Eng 2022,10, 6794-6806. Read online

162. Fryer, T.; Rogers, J. D.; Mellor, C.; Kohler, T. N.; Miner, R.; Hollfelder, F. Gigavalent Display of Proteins on Monodisperse Polyacrylamide Hydrogels as a Versatile Modular Platform for Functional Assays and Protein Engineering. ACS Cent Sci 2022, 8, 1182-1195 Read online.


161. Lindenburg, L. H.; Pantelejevs, T.; Gielen, F.; Zuazua-Villar, P.; Butz, M.; Rees, E.; Kaminski, C. F.; Downs, J. A.; Hyvonen, M.; Hollfelder, F. Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats. Proc Natl Acad Sci U S A, 2021, 118 (46), 2017708118. Read online

160. Klaus, M.; Zurek, P. J.; Kaminski, T. S.; Pushpanath, A.; Neufeld, K.; Hollfelder, F. Ultrahigh-Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay. Chembiochem 2021, 22, 3292-3299. Read online

159. Zurek, P. J.; Hours, R.; Schell, U.; Pushpanath, A.; Hollfelder, F. Growth amplification in ultrahigh-throughput microdroplet screening increases sensitivity of clonal enzyme assays and minimizes phenotypic variation. Lab Chip 2021, 21, 163-173.Read online

158. Schindler, M.; Siriwardena, D.; Kohler, T. N.; Ellermann, A. L.; Slatery, E.; Munger, C.; Hollfelder, F.; Boroviak, T. E. Agarose microgel culture delineates lumenogenesis in naive and primed human pluripotent stem cells. Stem Cell Reports 2021, 16, 1347-1362.Read online

157. Schenkmayerova, A.; Pinto, G. P.; Toul, M.; Marek, M.; Hernychova, L.; Planas-Iglesias, J.; Daniel Liskova, V.; Pluskal, D.; Vasina, M.; Emond, S.; Dorr, M.; Chaloupkova, R.; Bednar, D.; Prokop, Z.; Hollfelder, F.; Bornscheuer, U. T.; Damborsky, J. Engineering the protein dynamics of an ancestral luciferase. Nat Commun 2021, 12, 3616. Read online

156. Lindenburg, L.; Hollfelder, F. "NAD-display": Ultrahigh-Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry. Angew Chem Int Ed Engl 2021, 60, 9015-9021. Read online

155. Knyphausen, P.; Lindenburg, L.; Hollfelder, F. Error-Free Synthetic DNA by Molecular Dictation. Trends Biotechnol 2021, 39, 861-865. Read online

154. Huovinen, T.; Lindenburg, L.; Minter, R.; Hollfelder, F. Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays. Anal Chem 2021, 93, 2166-2173. Read online

153. Holstein, J. M.; Gylstorff, C.; Hollfelder, F. Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput. ACS Synth Biol 2021, 10, 252-257. Read online

152. Cordero-Espinoza, L.; Dowbaj, A. M.; Kohler, T. N.; Strauss, B.; Sarlidou, O.; Belenguer, G.; Pacini, C.; Martins, N. P.; Dobie, R.; Wilson-Kanamori, J. R.; Butler, R.; Prior, N.; Serup, P.; Jug, F.; Henderson, N. C.; Hollfelder, F.; Huch, M. Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation. Cell Stem Cell 2021,  28 (11), 907-1921.e8. Read online

151. Biggs, G. S.; Klein, O. J.; Maslen, S. L.; Skehel, J. M.; Rutherford, T. J.; Freund, S. M. V.; Hollfelder, F.; Boss, S. R.; Barker, P. D. Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naive Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation. Angew Chem Int Ed Engl 2021, 60, 10919-10927. Read online

150.  Amadei, G.; Lau, K. Y. C.; De Jonghe, J.; Gantner, C. W.; Sozen, B.; Chan, C.; Zhu, M.; Kyprianou, C.; Hollfelder, F.; Zernicka-Goetz, M. Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro. Dev Cell 2021, 56, 366-382 e369. Read online

149. Alex Wong, C. F.; van Vliet, L.; Bhujbal, S. V.; Guo, C.; Sletmoen, M.; Stokke, B. T.; Hollfelder, F.; Lale, R. A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution. ACS Synth Biol 2021, 10, 1882-1894. Read online


2020(back to top)

148.  Zurek, P. J.; Knyphausen, P.; Neufeld, K.; Pushpanath, A.; Hollfelder, F. UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution. Nat Commun 2020, 11, 6023. Read online

147.  Tauzin, A. S.; Pereira, M. R.; Van Vliet, L. D.; Colin, P. Y.; Laville, E.; Esque, J.; Laguerre, S.; Henrissat, B.; Terrapon, N.; Lombard, V.; Leclerc, M.; Dore, J.; Hollfelder, F.; Potocki-Veronese, G. Investigating host-microbiome interactions by droplet based microfluidics. Microbiome 2020, 8, 141. Read online

146. Skamaki, K.; Emond, S.; Chodorge, M.; Andrews, J.; Rees, D. G.; Cannon, D.; Popovic, B.; Buchanan, A.; Minter, R. R.; Hollfelder, F. In vitro evolution of antibody affinity via insertional scanning mutagenesis of an entire antibody variable region. Proc Natl Acad Sci U S A 2020, 117, 27307-27318. Read online

145.  Neun, S.; Zurek, P. J.; Kaminski, T. S.; Hollfelder, F.: Ultrahigh throughput screening for enzyme function in droplets. In Enzyme Engineering and Evolution: General Methods; Tawfik, D. S., Ed.; Methods in Enzymology; Academic Press Ltd-Elsevier Science Ltd: London, 2020; Vol. 643; pp 317-343. Read online

144. Mulas, C.; Hodgson, A. C.; Kohler, T. N.; Agley, C. C.; Humphreys, P.; Kleine-Bruggeney, H.; Hollfelder, F.; Smith, A.; Chalut, K. J. Microfluidic platform for 3D cell culture with live imaging and clone retrieval. Lab Chip 2020, 20, 2580-2591. Read online

143. Lindenburg, L.; Huovinen, T.; van de Wiel, K.; Herger, M.; Snaith, M. R.; Hollfelder, F. Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins. Nucleic Acids Res 2020, 48, e63. Read online

142. Kuo, S. T.; Jahn, R. L.; Cheng, Y. J.; Chen, Y. L.; Lee, Y. J.; Hollfelder, F.; Wen, J. D.; Chou, H. D. Global fitness landscapes of the Shine-Dalgarno sequence. Genome Res 2020, 30, 711-723. Read online

141. Emond, S.; Petek, M.; Kay, E. J.; Heames, B.; Devenish, S. R. A.; Tokuriki, N.; Hollfelder, F. Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis. Nat Commun 2020, 11, 3469. Read online

140. Anagnostidis, V.; Sherlock, B.; Metz, J.; Mair, P.; Hollfelder, F.; Gielen, F. Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures. Lab Chip 2020, 20, 889-900. Read online


2019(back to top)

139. van Loo, B.; Heberlein, M.; Mair, P.; Zinchenko, A.; Schuurmann, J.; Eenink, B. D. G.; Holstein, J. M.; Dilkaute, C.; Jose, J.; Hollfelder, F.; Bornberg-Bauer, E. High-Throughput, Lysis-Free Screening for Sulfatase Activity Using Escherichia coli Autodisplay in Microdroplets. ACS Synth Biol 2019, 8, 2690-2700. Read online

138. van Loo, B.; Berry, R.; Boonyuen, U.; Mohamed, M. F.; Golicnik, M.; Hengge, A. C.; Hollfelder, F. Transition-State Interactions in a Promiscuous Enzyme: Sulfate and Phosphate Monoester Hydrolysis by Pseudomonas aeruginosa Arylsulfatase. Biochemistry 2019, 58, 1363-1378. Read online

137. van Loo, B.; Bayer, C. D.; Fischer, G.; Jonas, S.; Valkov, E.; Mohamed, M. F.; Vorobieva, A.; Dutruel, C.; Hyvonen, M.; Hollfelder, F. Balancing Specificity and Promiscuity in Enzyme Evolution: Multidimensional Activity Transitions in the Alkaline Phosphatase Superfamily. J Am Chem Soc 2019, 141, 370-387. Read online

136. Sozen, B.; Cox, A. L.; De Jonghe, J.; Bao, M.; Hollfelder, F.; Glover, D. M.; Zernicka-Goetz, M. Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid. Dev Cell 2019, 51, 698-712 e698. Read online

135. Schenkmayerova, A.; Pinto, G.; Marek, M.; Toul, M.; Hernychova, L.; Liskova, V.; Emond, S.; Bednar, D.; Prokop, Z.; Chaloupkova, R.; Hollfelder, F.; Bornscheuer, U. T. Functional switching based on altered enzyme flexibility via InDel mutagenesis of a reconstructed ancestor. J. Biotechnol. 2019, 305, S32-S32. Read online

134. Neun, S.; Kaminski, T. S.; Hollfelder, F.: Single-cell activity screening in microfluidic droplets. In Enzyme Activity in Single Cells; Allbritton, N. L., Kovarik, M. L., Eds.; Methods in Enzymology; Academic Press Ltd-Elsevier Science Ltd: London, 2019; Vol. 628; pp 95-112. Read online

133.  Kleine-Bruggeney, H.; van Vliet, L. D.; Mulas, C.; Gielen, F.; Agley, C. C.; Silva, J. C. R.; Smith, A.; Chalut, K.; Hollfelder, F. Long-Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation. Small 2019, 15, e1804576. Read online

132.  Buryska, T.; Vasina, M.; Gielen, F.; Vanacek, P.; van Vliet, L.; Jezek, J.; Pilat, Z.; Zemanek, P.; Damborsky, J.; Hollfelder, F.; Prokop, Z. Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics. Anal Chem 2019, 91, 10008-10015. Read online


2018(back to top)

131. van Loo, B.; Schober, M.; Valkov, E.; Heberlein, M.; Bornberg-Bauer, E.; Faber, K.; Hyvonen, M.; Hollfelder, F. Structural and Mechanistic Analysis of the Choline Sulfatase from &ITSinorhizobium melliloti&IT: A Class I Sulfatase Specific for an Alkyl Sulfate Ester. J. Mol. Biol. 2018, 430, 1004-1023. Read online

130. Schenkmayerova, A.; Pinto, G.; Liskova, V.; Emond, S.; Hernychova, L.; Bednar, D.; Chaloupkova, R.; Hollfelder, F.; Damborsky, J. Modification of protein dynamics by directed evolution based on insertions and deletions. FEBS Open Bio 2018, 8, 400-400. Read online

129. Saher O, Rocha CSJ, Zaghloul EM, Wiklander OPB, Zamolo S, Heitz M, Ezzat K, Gupta D, Reymond JL, Zain R, Hollfelder F, Darbre T, Lundin KE, El Andaloussi S, Smith CIE. Novel peptide-dendrimer/lipid/oligonucleotide ternary complexes for efficient cellular uptake and improved splice-switching activity. Eur J Pharm Biopharm. 2018;132:29-40. Read online

128. Petek, M.; Emond, S.; Hollfelder, F. Recording the Fitness Landscapes of Small Deletions and Substitutions in the Green Fluorescent Protein. Protein Sci. 2018, 27, 214-215. Read online

127. van Loo, B.; Schober, M.; Valkov, E.; Heberlein, M.; Bornberg-Bauer, E.; Faber, K.; Hyvönen, M.; Hollfelder, F. Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti: A Class I Sulfatase Specific for an Alkyl Sulfate Ester. Journal of Molecular Biology 2018, 430(7): 1004-1023. Read online

126. Miton, C. M.; Jonas, S.; Fischer, G.; Duarte, F.; Mohamed, M. F.; van Loo, B.; Kintes, B.; Kamerlin, S. C. L.; Tokuriki, N.; Hyvönen, M.; Hollfelder, F. Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset. Proc. Natl Acad. Sci USA 2018, 201607817. Read online

125. Gielen, F.; Colin, P.Y.; Mair, P.; Hollfelder, F. Ultrahigh-Throughput Screening of Single-Cell Lysates for Directed Evolution and Functional Metagenomics. Methods Mol Biol. 2018, 1685:297-309. Read online

124. Bilsland, E.; van Vliet, L.; Williams, K.; Feltham, J.; Carrasco, M.P.; Fotoran, W.L.; Cubillos, E.F.G.; Wunderlich, G; Grøtli, M.; Hollfelder, F.; Jackson, V.; King, R.D.; Oliver, S.G. Plasmodium dihydrofolate reductase is a second enzyme target for the antimalarial action of triclosan. Scientific Reports 2018, 8(1):1038. Read online


2017(back to top)

123. Sum, T.H.; Sum, T.J.; Collins, S.; Galloway, W.R.J.D.; Twigg, D.G.; Hollfelder, F.; Spring, D.R., Divergent synthesis of biflavonoids yields novel inhibitors of the aggregation of amyloid β (1-42). Org. Biomol. Chem. 201715, 4554-4570 . Read online

122. Mair, P.; Gielen, F.; Hollfelder, F., Exploring sequence space in search of functional enzymes using microfluidic droplets. Curr Opin Chem Biol 2017, 37, 137-44. Read online

121. Heitz, M.; Kwok, A.; Eggimann, G.A.; Hollfelder, F.; Darbre, T.; Reymond, J.L., Peptide Dendrimer-Lipid Conjugates as DNA and siRNA Transfection Reagents: Role of Charge Distribution Across Generations. CHIMIA International Journal for Chemistry 2017, 71(4), 220-225. Read online

120. Gielen, F.; Butz, M.; Rees, E.J.; Erdelyi, M.; Moschetti, T.; Hyvönen, M.; Edel, J.B.; Kaminski, C.F.; Hollfelder, F., Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets. Anal Chem. 2017, 89(2), 1092-1101. Read online

119. Collins, S.; Gielen, F.; van Vliet, L.; Kaminski, G.; Hollfelder, F.; Spring, D. Identification and development of small molecule inhibitors of the aggregation of amyloid beta. Abstr. Pap. Am. Chem. Soc. 2017, 253, 2.

118. Bayer, C.D.; van Loo, B.; Hollfelder, F., Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context-Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Space. ChemBiochem 2017, 18(1), 1001–1015. Read online


2016(back to top)

117. Sum, T.H.; Sum, T.J.; Galloway, W.R.; Collins, S.; Twigg, D.G.; Hollfelder, F; Spring, D.R., Combinatorial Synthesis of Structurally Diverse Triazole-Bridged Flavonoid Dimers and Trimers. Molecules 2016, 21(9), 1230. Read online

116. Mankowska, S.A.; Gatti-Lafranconi, P.; Chodorge, M.; Sridharan, S.; Minter, R.R.; Hollfelder, F., A Shorter Route to Antibody Binders via Quantitative in vitro Bead-Display Screening and Consensus Analysis. Scientific Reports 2016, 6: 36391. Read online

115. Kwok, A.; Eggimann, G.A.; Heitz, M.; Reymond, J. L.; Hollfelder, F.; Darbre, T., Efficient Transfection of siRNA by Peptide Dendrimer–Lipid Conjugates. ChemBioChem 2016, 17(23), 2223–2229. Read online

114. Kaltenbach, M.; Emond, S.; Hollfelder, F.; Tokuriki, N., Functional trade-offs in promiscuous enzymes cannot be explained by intrinsic mutational robustness of the native activity. PLOS Genetics 2016, 12(10): e1006305. Read online

113. Gielen, F.; Hours, R.; Emond, S.; Fischlechner, M.; Schell, U.; Hollfelder, F., Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS). Proc. Natl Acad. Sci USA 2016113(47), E7383-E7389. Read online

112. Campbell, E. C.; Kaltenbach, M.; Correy, G.J.; Carr, P.D.; Porebski, B.T.; Livingstone, E.K.; Afriat-Jurnou, L.; Buckle, A.M.; Weik, M.; Hollfelder, F.; Tokuriki, N.; Jackson, C.J., The role of protein dynamics in the evolution of new enzyme function. Nature Chemical Biology 2016, 12(11), 944-950. Read online

111. Bayer, C. D.; Hollfelder, F.: A Short Practical Guide to the Quantitative Analysis of Engineered Enzymes; Pan Stanford Publishing Pte Ltd: Singapore, 2016. Link

2015(back to top)

110. van Vliet, L. D.; Colin, P.Y.; Hollfelder, F., Bioinspired genotype–phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins.  Interface Focus 2015, 5(4), 20150035. Read online

109. Urbach, C.; Gordon, N.C.; Strickland, I.; Lowne, D.; Joberty-Candotti, C.; May, R.; Herath, A.; Hijnen, D.; Thijs, J.L.; Bruijnzeel-Koomen C.A.; Minter, R.R.; Hollfelder, F.; Jermutus, L., Combinatorial Screening Identifies Novel Promiscuous Matrix Metalloproteinase Activities that Lead to Inhibition of the Therapeutic Target IL-13. Chem Biol 2015, 22(11), 1442-1452. Read online

108. Shafee, T.; Gatti-Lafranconi, P.; Minter, R.; Hollfelder, F., Handicap-recover evolution leads to a chemically versatile, nucleophile-permissive protease. ChemBioChem 2015, 16(13), 1866–1869. Read online

107. Kaltenbach, M.; Jackson, C.J.; Campbell, E. C.; Hollfelder, F., Tokuriki, N., Reverse evolution leads to genotypic incompatibility despite functional and active-site convergence. Elife 2015, eLife06492. Read online

106. Houlihan, G.; Gatti-Lafranconi, P.; Lowe, D.; Hollfelder, F., Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process. Protein Eng Des Sel 2015, 28(9), 269-279. Read online

105. Gielen, F.; Buryska, T.; Van Vliet ,L.; Butz, M.; Damborsky, J.; Prokop, Z.; Hollfelder, F., Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets. Anal. Chem. 2015, 87(1), 624-32. Read online

104. Fradet, E.; Bayer, C.D.; Hollfelder, F.; Baroud, C.N., Measuring fast and slow enzyme kinetics in stationary droplets. Anal. Chem. 201587(23), 11915–11922. Read online

103. Colin, P.Y.; Zinchenko, A.; Hollfelder, F., Enzyme engineering in biomimetic compartments. Curr Opin Struct Biol 2015, 33, 42-51. Read online

102. Colin, P.Y.; Kintses, B.; Gielen, F.; Miton, C.M.; Fischer, G.; Mohamed, M.F.; Hyvönen, M.; Morgavi, D.P.; Janssen, D.B.; Hollfelder, F., Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nature Communications 2015, 6:10008. Read online

101. Chen, W.; Avezov, E.; Schlachter, S. C.; Gielen, F.; Laine, R. F.; Harding, H. P.; Hollfelder, F.; Ron, D.; Kaminski, C. F., A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime in growth. Biophys J. 2015, 108(5), 999-1002. Read online

2014 (back to top)

100. Zinchenko, A.; Devenish, S. R.; Kintses, B.; Colin, P. Y.; Fischlechner, M.; Hollfelder, F., One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution. Anal Chem 2014, 86 (5), 2526-33. Read online

99. Villiers, B.; Hollfelder, F., USER friendly DNA recombination (USERec): gene library construction requiring minimal sequence homology. Methods Mol Biol 2014, 1179, 213-24. Read online

98. Houlihan, G.; Gatti-Lafranconi, P.; Kaltenbach, M.; Lowe, D.; Hollfelder, F., An experimental framework for improved selection of binding proteins using SNAP display. J Immunol Methods 2014,  (405), 47-56. Read online

97. Fischlechner, M.; Schaerli, Y.; Mohamed, M. F.; Patil, S.; Abell, C.; Hollfelder, F., Evolution of enzyme catalysts caged in biomimetic gel-shell beads. Nat Chem 2014, 6 (9), 791-6. Read online

2013 (back to top)

96. Yan, J.; Bauer, W. A. C.; Fischlechner, M.; Hollfelder, F.; Kaminski, C. F.; Huck, W. T. S., Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry. Micromachines 2013, 4 (4), 402-413. Read online

95. Shim, J. U.; Ranasinghe, R. T.; Smith, C. A.; Ibrahim, S. M.; Hollfelder, F.; Huck, W. T.; Klenerman, D.; Abell, C., Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays. ACS Nano 2013, 7 (7), 5955-64. Read online

94. Schober, M.; Toesch, M.; Knaus, T.; Strohmeier, G. A.; van Loo, B.; Fuchs, M.; Hollfelder, F.; Macheroux, P.; Faber, K., One-pot deracemization of sec-alcohols: enantioconvergent enzymatic hydrolysis of alkyl sulfates using stereocomplementary sulfatases. Angew Chem Int Ed Engl 2013, 52 (11), 3277-9. Read online

93. Mohamed, M. F.; Hollfelder, F., Efficient, crosswise catalytic promiscuity among enzymes that catalyze phosphoryl transfer. Biochim Biophys Acta 2013, 1834 (1), 417-24. Read online

92. Kwok, A.; Eggimann, G. A.; Reymond, J. L.; Darbre, T.; Hollfelder, F., Peptide dendrimer/lipid hybrid systems are efficient DNA transfection reagents: structure--activity relationships highlight the role of charge distribution across dendrimer generations. ACS Nano 2013, 7 (5), 4668-82. Read online

91. Houlihan, G.; Lowe, D.; Hollfelder, F., SNAP display - an in vitro method for the selection of protein binders. Curr Pharm Des 2013, 19 (30), 5421-8. Read online

90. Houlihan, G.; Gatti-Lafranconi, P.; Kaltenbach, M.; Lowe, D.; Hollfelder, F. In vitro selection and evolution of DARPins using SNAP display. Febs Journal 2013, 280, 471-471. Read online

89. Hollfelder, F.; Lutz, S., Just (protein) engineering? Curr Opin Struct Biol 2013, 23 (4), 569-70. Read online

88. Hollfelder, F. Multiple Catalytic Promiscuity: Specificity and Efficiency in the Alkaline Phosphatase Superfamily. Faseb J. 2013, 27, 1.

87. Goldsworthy, M.; Absalom, N. L.; Schroter, D.; Matthews, H. C.; Bogani, D.; Moir, L.; Long, A.; Church, C.; Hugill, A.; Anstee, Q. M.; Goldin, R.; Thursz, M.; Hollfelder, F.; Cox, R. D., Mutations in Mll2, an H3K4 methyltransferase, result in insulin resistance and impaired glucose tolerance in mice. PLoS One 2013, 8 (6), e61870. Read online

86. Gielen, F.; van Vliet, L.; Koprowski, B. T.; Devenish, S. R.; Fischlechner, M.; Edel, J. B.; Niu, X.; deMello, A. J.; Hollfelder, F., A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition. Anal Chem 2013, 85 (9), 4761-9. Read online

85. Gatti-Lafranconi, P.; Hollfelder, F., Flexibility and reactivity in promiscuous enzymes. Chembiochem 2013, 14 (3), 285-92. Read online

84. Gatti-Lafranconi, P.; Dijkman, W. P.; Devenish, S. R.; Hollfelder, F., A single mutation in the core domain of the lac repressor reduces leakiness. Microb Cell Fact 2013, 12, 67. Read online

83. Diamante, L.; Gatti-Lafranconi, P.; Schaerli, Y.; Hollfelder, F., In vitro affinity screening of protein and peptide binders by megavalent bead surface display. Protein Eng Des Sel 2013, 26 (10), 713-24. Read online

82. Devenish, S. R.; Kaltenbach, M.; Fischlechner, M.; Hollfelder, F., Droplets as reaction compartments for protein nanotechnology. Methods Mol Biol 2013, 996, 269-86. Read online

2012 (back to top)

81. Stein, V.; Kaltenbach, M.; Hollfelder, F., Assembling linear DNA templates for in vitro transcription and translation. Methods Mol Biol (Functional Genomics: Methods and Protocols) 2012, 815, 67-78. Read online

80. Schaerli, Y.; Kintses, B.; Hollfelder, F., Protein Engineering in Microdroplets. In Protein Engineering Handbook, Lutz, S.; Bornscheuer, U. T., Eds. Wiley VCH: Weinheim, 2012; Vol. 3, pp 73-85. Link

79. Miton, C.; Jonas, S.; Hyvonen, M.; Tokuriki, N.; Hollfelder, F. Exploring Catalytic Promiscuity in the Alkaline Phosphatase Superfamily by Directed Evolution. Protein Sci. 2012, 21, 135-135.

78. Kintses, B.; Hein, C.; Mohamed, M. F.; Fischlechner, M.; Courtois, F.; Laine, C.; Hollfelder, F., Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution. Chem Biol 2012, 19 (8), 1001-9. Read online

77. Kaltenbach, M.; Hollfelder, F., SNAP display: in vitro protein evolution in microdroplets. Methods Mol Biol (Ribosome Display and Related Technologies: Methods and Protocols) 2012, 805, 101-11. Read online

76. Kaltenbach, M.; Devenish, S. R.; Hollfelder, F., A simple method to evaluate the biochemical compatibility of oil/surfactant mixtures for experiments in microdroplets. Lab Chip 2012, 12 (20), 4185-92. Read online

75. Hollfelder, F. Rules and tools for directed evolution. New Biotech. 2012, 29, S8-S8. Read online

74. Emond, S.; Tokuriki, N.; Hollfelder, F. New tools for random insertion and deletion mutagenesis in directed evolution. Protein Sci. 2012, 21, 184-185.

73. Diamante, L.; Gatti-Lafranconi, P.; Hollfelder, F. Bead surface display for affinity screening of protein and peptide binders. New Biotech. 2012, 29, S35-S36. Read online

72. Delley, R. J.; Bandyopadhyay, S.; Fox, M. A.; Schliehe, C.; Hodgson, D. R.; Hollfelder, F.; Kirby, A. J.; O'Donoghue, A. C., peri-Dimethylamino substituent effects on proton transfer at carbon in alpha-naphthylacetate esters: a model for mandelate racemase. Org Biomol Chem 2012, 10 (3), 590-6. Read online

71. Babtie, A. C.; Lima, M. F.; Kirby, A. J.; Hollfelder, F., Kinetic and computational evidence for an intermediate in the hydrolysis of sulfonate esters. Org Biomol Chem 201210, 8095-8101. Read online    

2011 (back to top)

70. Villiers, B.; Hollfelder, F., Directed evolution of a gatekeeper domain in nonribosomal peptide synthesis. Chem Biol 2011, 18 (10), 1290-9. Read online

69. Shim, J. U.; Patil, S. N.; Hodgkinson, J. T.; Bowden, S. D.; Spring, D. R.; Welch, M.; Huck, W. T.; Hollfelder, F.; Abell, C., Controlling the contents of microdroplets by exploiting the permeability of PDMS. Lab Chip 2011, 11(6), 1132-7. Read online

68. Kaltenbach, M.; Stein, V.; Hollfelder, F., SNAP dendrimers: multivalent protein display on dendrimer-like DNA for directed evolution. Chembiochem 2011, 12 (14), 2208-16. Read online

67. Huebner, A. M.; Abell, C.; Huck, W. T.; Baroud, C. N.; Hollfelder, F., Monitoring a reaction at submillisecond resolution in picoliter volumes. Anal Chem 2011, 83 (4), 1462-8. Read online

66. Hollfelder, F., From fascination to function. Curr Opin Biotechnol 2011, 22 (4), 473-4. Read online

2010 (back to top)

65. Villiers, B. R.; Stein, V.; Hollfelder, F., USER friendly DNA recombination (USERec): a simple and flexible near homology-independent method for gene library construction. Protein Eng Des Sel 2010, 23 (1), 1-8. Read online

64. van Loo, B.; Jonas, S.; Babtie, A. C.; Benjdia, A.; Berteau, O.; Hyvönen, M.; Hollfelder, F., An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily. Proc. Natl Acad. Sci USA 2010, 107 (7), 2740-5. Read online

63. van Loo, B.; Hollfelder, F., Enzyme promiscuity and evolution of new protein functions. In Manual of Industrial Microbiology and Biotechnology, 3rd ed.; Zhao, H., Ed. American Society for Microbiology: Washington, 2010; Vol. 3rd, pp 524-538. Read online

62. Theberge, A. B.; Courtois, F.; Schaerli, Y.; Fischlechner, M.; Abell, C.; Hollfelder, F.; Huck, W. T., Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. Angew Chem Int Ed Engl 2010, 49 (34), 5846-68. Read online

61. Schaerli, Y.; Stein, V.; Spiering, M. M.; Benkovic, S. J.; Abell, C.; Hollfelder, F., Isothermal DNA amplification using the T4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification. Nucleic Acids Res 2010, 38 (22), e201. Read online

60. O’Brien, P. J.; Hollfelder, F., Hitting a moving target?—understanding how conformational diversity impacts enzymatic catalysis. Curr. Op. Chem. Biol. 2010, 14 (5), 634-5. Read online

59. Kintses, B.; van Vliet, L. D.; Devenish, S. R.; Hollfelder, F., Microfluidic droplets: new integrated workflows for biological experiments. Curr Opin Chem Biol 2010, 14 (5), 548-55. Read online

58. Bai, Y.; He, X.; Liu, D.; Patil, S. N.; Bratton, D.; Huebner, A.; Hollfelder, F.; Abell, C.; Huck, W. T. S., A double droplet trap system for studying mass transport across a droplet-droplet interface. Lab Chip 2010, 10 (10), 1281-5. Read online

57. Babtie, A. C.; Tokuriki, N.; Hollfelder, F., What makes an enzyme promiscuous? Curr. Op. Chem. Biol 2010, 14 (2), 200-7. Read online

2009 (back to top)

56. Villiers, B. R. M.; Hollfelder, F., Mapping the Limits of Substrate Specificity of the Adenylation Domain of TycA. Chembiochem 2009, 10 (4), 671-682. Read online

55. Stein, V.; Hollfelder, F., An efficient method to assemble linear DNA templates for in vitro screening and selection systems. Nucleic Acids Res 2009, 37 (18), e122. Read online

54. Shim, J. U.; Olguin, L. F.; Whyte, G.; Scott, D.; Babtie, A.; Abell, C.; Huck, W. T.; Hollfelder, F., Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments. J Am Chem Soc 2009, 131 (42), 15251-6. Read online

53. Schaerli, Y.; Wootton, R. C.; Robinson, T.; Stein, V.; Dunsby, C.; Neil, M. A. A.; French, P. M. W.; deMello, A. J.; Abell, C.; Hollfelder, F., Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets. Analytical Chemistry 2009, 81 (1), 302-306. Read online

52. Schaerli, Y.; Hollfelder, F., The potential of microfluidic water-in-oil droplets in experimental biology. Mol Bio Sys 2009, 5 (12), 1392-404. Read online

51. Robinson, T.; Schaerli, Y.; Wootton, R.; Hollfelder, F.; Dunsby, C.; Baldwin, G.; Neil, M.; French, P.; Demello, A., Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging. Lab Chip 2009, 9 (23), 3437-41. Read online

50. Kirby, A. J.; Hollfelder, F., From Enzyme Models to Model Enzymes. Royal Society of Chemistry Cambridge, 2009; p 228. Read online

49. Kaltenbach, M.; Schärli, Y.; Hollfelder, F., Microdroplets - A Tool for Protein Engineering. BioForum 2009, 13, 19-21.

48. Jonas, S.; Hollfelder, F., Mapping catalytic promiscuity in the alkaline phosphatase superfamily. Pure and Applied Chemistry 2009, 81 (4), 731-742. Read online

47. Hufnagel, H.; Huebner, A.; Gulch, C.; Guse, K.; Abell, C.; Hollfelder, F., An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets. Lab on a Chip 2009, 9 (11), 1576-1582. Read online

46. Hufnagel, H.; Hakim, P.; Lima, A.; Hollfelder, F., Fluid Phase Endocytosis Contributes to Transfection of DNA by PEI-25. Mol. Therapy 2009, 17 (8), 1411-7. Read online

45. Huebner, A.; Bratton, D.; Whyte, G.; Yang, M.; deMello, A. J.; Abell, C.; Hollfelder, F., Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays. Lab on a Chip 2009, 9 (5), 692-698. Read online

44. Golicnik, M.; Olguin, L. F.; Feng, G.; Baxter, N. J.; Waltho, J. P.; Williams, N. H.; Hollfelder, F., Kinetic analysis of beta-phosphoglucomutase and its inhibition by magnesium fluoride. J Am Chem Soc 2009, 131 (4), 1575-88. Read online

43. Damean, N.; Olguin, L. F.; Hollfelder, F.; Abell, C.; Huck, W. T. S., Simultaneous measurement of reactions in microdroplets filled by concentration gradients. Lab on a Chip 2009, 9 (12), 1707-1713. Read online

42. Courtois, F.; Olguin, L. F.; Whyte, G.; Theberge, A. B.; Huck, W. T. S.; Hollfelder, F.; Abell, C., Controlling the Retention of Small Molecules in Emulsion Microdroplets for Use in Cell-Based Assays. Analytical Chemistry 2009, 81 (8), 3008-3016. Read online

41.Courtois, F.; Huebner, A.; Stein, V.; Olguin, L.; Hollfelder, F., Towards biological experimentation in microfluidic microdroplets. La Houille Blanche 2009, 5, 127-133. Read online

40. Babtie, A. C.; Bandyopadhyay, S.; Olguin, L. F.; Hollfelder, F., Efficient Catalytic Promiscuity for Chemically Distinct Reactions. Angewandte Chemie-International Edition 2009, 48 (20), 3692-3694. Read online

39. Avenier, F.; Hollfelder, F., Combining Medium Effects and Cofactor Catalysis: Metal-Coordinated Synzymes Accelerate Phosphate Transfer by 10(8). Chemistry-a European Journal 2009, 15 (45), 12371-12380. Read online

2008 (back to top)

38. Van Vliet, L. D.; Chapman, M. R.; Avenier, F.; Kitson, C. Z.; Hollfelder, F., Relating chemical and biological diversity space: A tunable system for efficient gene transfection. Chembiochem 2008, 9 (12), 1960-1967. Read online

37. Shim, J. U.; Olguin, L.; Hollfelder, F.; Abell, C.; Huck, W., Cell based biological assays using microfluidics. Ekc2008: Proceedings of the Eu-Korea Conference on Science and Technology 2008, 124, 499-502. Read online

36. Olguin, L. F.; Askew, S. E.; O'Donoghue, A. C.; Hollfelder, F., Efficient Catalytic Promiscuity in an Enzyme Superfamily: An Arylsulfatase Shows a Rate Acceleration of 10(13) for Phosphate Monoester Hydrolysis. Journal of the American Chemical Society 2008, 130 (49), 16547-16555. Read online

35. Leemhuis, H.; Packman, L. C.; Nightingale, K. P.; Hollfelder, F., The human histone acetyltransferase P/CAF is a promiscuous histone propionyltransferase. Chembiochem 2008, 9 (4), 499-503. Read online

34. Leemhuis, H.; Nightingale, K. P.; Hollfelder, F., Directed evolution of a histone acetyltransferase - enhancing thermostability, whilst maintaining catalytic activity and substrate specificity. The FEBS Journal 2008, 275 (22), 5635-5647. Read online

33. Kirby, A. J.; Hollfelder, F., Biochemistry: Enzymes under the nanoscope. Nature 2008, 456 (7218), 45. Read online

32. Jonas, S.; van Loo, B.; Hyvonen, M.; Hollfelder, F., A New Member of the Alkaline Phosphatase Superfamily with a Formylglycine Nucleophile: Structural and Kinetic Characterisation of a Phosphonate Monoester Hydrolase/Phosphodiesterase from Rhizobium leguminosarum. Journal of Molecular Biology 2008, 384 (1), 120-136. Read online

31. Jonas, S.; Hollfelder, F., Mechanism and Catalytic Promiscuity: Emerging Mechanistic Principles for Identification and Manipulation of Catalytically Promiscuous Enzymes. In The Handbook of Protein Engineering, Bornscheuer, U. T.; Lutz, S., Eds. Wiley-VCH: Chichester, 2008; Vol. 1, pp 47–72. Read online

30. Huebner, A.; Sharma, S.; Srisa-Art, M.; Hollfelder, F.; Edel, J. B.; Demello, A. J., Microdroplets: A sea of applications? Lab on a Chip 2008, 8 (8), 1244-1254. Read online

29. Huebner, A.; Olguin, L. F.; Bratton, D.; Whyte, G.; Huck, W. T. S.; de Mello, A. J.; Edel, J. B.; Abell, C.; Hollfelder, F., Development of quantitative cell-based enzyme assays in microdroplets. Analytical Chemistry 2008, 80 (10), 3890-3896. Read online

28. Courtois, F.; Olguin, L. F.; Whyte, G.; Bratton, D.; Huck, W. T. S.; Abell, C.; Hollfelder, F., An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets. Chembiochem 2008, 9 (3), 439-446. Read online

27. Baxter, N. J.; Blackburn, G. M.; Marston, J. P.; Hounslow, A. M.; Cliff, M. J.; Bermel, W.; Williams, N. H.; Hollfelder, F.; Wemmer, D. E.; Waltho, J. P., Anionic charge is prioritized over geometry in aluminum and magnesium fluoride transition state analogs of phosphoryl transfer enzymes. Journal of the American Chemical Society 2008, 130 (12), 3952-3958. Read online

2007 (back to top)

26. Van Vliet, L. D.; Ellis, T.; Foley, P. J.; Liu, L. G.; Pfeffer, F. M.; Russell, R. A.; Warrener, R. N.; Hollfelder, F.; Waring, M. J., Molecular recognition of DNA by rigid [n]-polynorbornane-derived bifunctional intercalators: Synthesis and evaluation of their binding properties. Journal of Medicinal Chemistry 2007, 50 (10), 2326-2340. Read online

25. Stein, V.; Sielaff, I.; Johnsson, K.; Hollfelder, F., A covalent chemical genotype-phenotype linkage for in vitro protein evolution. Chembiochem 2007, 8 (18), 2191-2194. Read online

24. Otten, L. G.; Schaffer, M. L.; Villiers, B. R.; Stachelhaus, T.; Hollfelder, F., An optimized ATP/PP(i)-exchange assay in 96-well format for screening of adenylation domains for applications in combinatorial biosynthesis. Biotechnol J 2007, 2 (2), 232-40. Read online

23. Nightingale, K. P.; Gendreizig, S.; White, D. A.; Bradbury, C.; Hollfelder, F.; Turner, B. M., Cross-talk between histone modifications in response to histone deacetylase inhibitors - MLL4 links histone H3 acetylation and histone H3K4 methylation. Journal of Biological Chemistry 2007, 282 (7), 4408-4416. Read online

22. Huebner, A.; Srisa-Art, M.; Holt, D.; Abell, C.; Hollfelder, F.; Demello, A. J.; Edel, J. B., Quantitative detection of protein expression in single cells using droplet microfluidics. Chemical Communications 2007,  (12), 1218-1220. Read online

21. Avenier, F.; Domingos, J. B.; Van Vliet, L. D.; Hollfelder, F., Polyethylene imine derivatives ('Synzymes') accelerate phosphate transfer in the absence of metal. Journal of the American Chemical Society 2007, 129 (24), 7611-7619. Read online

2006 (back to top)

20. Mathonet, P.; Leemhuis, H.; Griffiths, A. D.; Hollfelder, F., Towards compartmentalised selections of protein-protein interactions using a split-protein sensor. FEBS Journal 2006, 273, 253-253.

19. Baxter, N. J.; Olguin, L. F.; Golicnik, M.; Feng, G.; Hounslow, A. M.; Bermel, W.; Blackburn, G. M.; Hollfelder, F.; Waltho, J. P.; Williams, N. H., A Trojan horse transition state analogue generated by MgF3- formation in an enzyme active site. Proceedings of the National Academy of Sciences of the United States of America 2006, 103 (40), 14732-14737. Read online

Before 2005 (back to top)

18. Leemhuis, H.; Stein, V.; Griffiths, A. D.; Hollfelder, F., New genotype-phenotype linkages for directed evolution of functional proteins. Current Opinion in Structural Biology 2005, 15 (4), 472-478. Read online

17. Kirby, A. J.; Gesser, J. C.; Hollfelder, F.; Priebe, J. P.; Nome, F., Intramolecular general acid catalysis of sulfate transfer - Nucleophilic attack by oxyanions on the SO3- group. Canadian Journal of Chemistry-Revue Canadienne De Chimie 2005, 83 (9), 1629-1636. Read online

16. Lindner, A.; Hollfelder, F., The Evolution of Catalysis. ChemBioChem 2004, 5, 241. Read online

15. Zamble, D. B.; Miller, D. A.; Heddle, J. G.; Maxwell, A.; Walsh, C. T.; Hollfelder, F., In vitro characterization of DNA gyrase inhibition by microcin B17 analogs with altered bisheterocyclic sites. Proceedings of the National Academy of Sciences of the United States of America 2001, 98 (14), 7712-7717. Read online

14. Hollfelder, F.; Kirby, A. J.; Tawfik, D. S., On the magnitude and specificity of medium effects in enzyme-like catalysts for proton transfer. Journal of Organic Chemistry 2001, 66 (17), 5866-5874. Read online

13. Heddle, J. G.; Blance, S. J.; Zamble, D. B.; Hollfelder, F.; Miller, D. A.; Wentzell, L. M.; Walsh, C. T.; Maxwell, A., The antibiotic microcin B17 is a DNA gyrase poison: Characterisation of the mode of inhibition. Journal of Molecular Biology 2001, 307 (5), 1223-1234. Read online

12. Perez-Juste, J.; Hollfelder, F.; Kirby, A. J.; Engberts, J. B. F. N., Vesicles accelerate proton transfer from carbon up to 850-fold. Organic Letters 2000, 2 (2), 127-130. Read online

11. Kirby, A. J.; Hollfelder, F.; Tawfik, D. S., Nonspecific catalysis by protein surfaces. Applied Biochemistry and Biotechnology 2000, 83 (1-3), 173-180. Read online

10. Hollfelder, F.; Kirby, A. J.; Tawfik, D. S.; Kikuchi, K.; Hilvert, D., Characterization of proton-transfer catalysis by serum albumins. Journal of the American Chemical Society 2000, 122 (6), 1022-1029. Read online

9. Zhang, Y. L.; Hollfelder, F.; Gordon, S. J.; Chen, L.; Keng, Y. F.; Wu, L.; Herschlag, D.; Zhang, Z. Y. Impaired transition state complementarity in the hydrolysis of O-arylphosphorothioates by protein-tyrosine phosphatases. Biochemistry 1999, 38, 12111-12123.

8. Hollfelder, F.; Kirby, A. J.; Tawfik, D. S., Efficient catalysis of proton transfer by synzymes. Journal of the American Chemical Society 1997, 119 (40), 9578-9579. Read online

7. Genre-Grandpierre, A.; Tellier, C.; Loirat, M. J.; Blanchard, D.; Hodgson, D. R. W.; Hollfelder, F.; Kirby, A. J., Catalysis of the Kemp elimination by antibodies elicited against a cationic hapten. Bioorganic & Medicinal Chemistry Letters 1997, 7 (19), 2497-2502. Read online

6. Hollfelder, F.; Kirby, A. J.; Tawfik, D. S., Off-the-shelf proteins that rival tailor-made antibodies as catalysts. Nature 1996, 383 (6595), 60-63. Read online

5. Hollfelder, F.; Herschlag, D., The Nature of the Transition-State for Enzyme-Catalyzed Phosphoryl Transfer - Hydrolysis of O-Aryl Phosphorothioates by Alkaline-Phosphatase. Biochemistry 1995, 34 (38), 12255-12264. Read online

4. Dalby, K. N.; Kirby, A. J.; Hollfelder, F., Simple but Efficient Models for Nuclease Catalysis. Pure and Applied Chemistry 1994, 66 (4), 687-694. Read online

3. Chandler, A. J.; Hollfelder, F.; Kirby, A. J.; Ocarroll, F.; Stromberg, R., Models for Enzyme-Catalyzed Phosphate Transfer - Comparisons of Reactivity Towards a Neighboring Hydroxy Group for Phosphodiester Anions and Acids - General Base Catalysis of the Cyclization of a Hydroxyalkyl Phosphate Triester. Journal of the Chemical Society-Perkin Transactions 2 1994,  (2), 327-333. Read online

2. Dalby, K. N.; Kirby, A. J.; Hollfelder, F., Models for Nuclease Catalysis - Mechanisms for General Acid Catalysis of the Rapid Intramolecular Displacement of Methoxide from a Phosphate Diester. Journal of the Chemical Society-Perkin Transactions 2 1993,  (7), 1269-1281. Read online

1. Dalby, K. N.; Hollfelder, F.; Kirby, A. J., Electrostatic Catalysis of the Hydrolysis of a Phosphate Diester in Water. Journal of the Chemical Society-Chemical Communications 1992,  (24), 1770-1771. Read online