PostDoc
Joined the lab: June 2016
Background and Current Projects: I graduated in Biological Sciences (B.S and B.A) at the São Paulo State University (Unesp), Brazil. My master's degree and Ph.D were obtained in Biotechnology at University of São Paulo (USP), Brazil. The topic of my Ph.D project was about functional metagenomics aiming at applications in biofuel, flavorings and bioremediation and led to the identification and characterization of new lipolytic enzymes. Part of my PhD work was done in collaboration with Marko Hyvönen at the University of Cambridge.
In my current project, metagenomic libraries from different environmental sources will be screened for lipases, laccases and proteases using high-throughput droplet-based assays. Hopefully, this work will lead to the identification and characterization of previously unrecorded biocatalysts that could prove useful for biotechnological applications.
Interests: watching movies, going to museums, enjoying the fresh air on a nice walk, passionate about paintings and tap dance.
Publications
[13] Knyphausen, P., Rangel Pereira, M., Brear, P., Hyvönen, M., Jermutus, L., & Hollfelder, F. (2023). Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture.. Nature communications, 14 (1). Read online
[12] Fernandes, G.C., Sierra, E.G.M., Paul Brear, Pereira, M.R. and Lemos, E.G.M..From Data Mining of Chitinophaga sp. Genome to Enzyme Discovery of a Hyperthermophilic Metallocarboxypeptidase. Microorganisms, v. ., p. 1-17, 2021. Read online
[11] Maester, T.C., Pereira, M.R., Malaman A.M.G, Borges, J.P, Pereira, P.A.M. and Lemos, E.G.M. Exploring Metagenomic Enzymes: A Novel Esterase Useful for Short-Chain Ester Synthesis. Catalysts, v. 10, p. 1100, 2020. Read online
[10] Tauzin, A.S., Pereira, M.R., Van Vliet, L.D., Colin, PY., Laville, E., Esque, J., Laguerre, S., Henrissat, B., Terrapon, N., Lombard, V., Leclerc, M., Doré, J., Hollfelder, F., Potocki-Veronese, G., Investigating host-microbiome interactions by droplet based microfluidics. Microbiome 8, 141 (2020). Read online.
[9] Sierra, E.M., Pereira, M.R., Maester, T.C., Gomes-Pepe, E.S., Mendoza, E.R., Lemos, E.G.M. Halotolerant aminopeptidase M29 from Mesorhizobium SEMIA 3007 with biotechnological potential and its impact on biofilm synthesis (2017). Sci Rep., 7(1):10684. Read online
[8] Maester, T.C., Gomes, E. S., Pereira, M.R., Machado Sierra, E. G., Lemos, M.V.F., Lemos, E.G.M. Bioprospecting Studies: Transforming the Natural Genetic Heritage into Biotechnological Richness (2017). In: The Brazilian Microbiome. Current Status and Perspectives, Editors: Pylro, V., Roesch, L., Springer (Cham), pp. 87-109. Read online
[7] Pereira, M.R., Maester, T.C., Mercaldi, G.F., Lemos, E.G.M., Hyvönen, M., Balan, A. From a metagenomic source to a high-resolution structure of a novel alkaline esterase (2017). Appl Microbiol Biotechnol., 101(12):4935-4949. Read online
[6] Banci, K.R., Mori, G.M., Oliveira, M.A., Paganelli, F.L., Pereira, M.R., Pinheiro, M.A. Can environmental pollution by metals change genetic diversity? Ucides cordatus (Linnaeus, 1763) as a study case in Southeastern Brazilian mangroves (2017). Mar Pollut Bull., 116(1-2):440-447. Read online
[5] Gomes-Pepe, E. S., Machado Sierra, E. G., Pereira, M. R., Castellane, T. C., Lemos, E. G. Bg10: A Novel Metagenomics Alcohol-Tolerant and Glucose-Stimulated GH1 ß-Glucosidase Suitable for Lactose-Free Milk Preparation (2016). PloS One, 11(12):e0167932. Read online
[4] Maester, T. C., Pereira, M. R., Sierra, E. G. M., Balan, A., Lemos, E. G. M. Characterization of EST3: A metagenome-derived esterase with suitable properties for biotechnological applications (2016). Applied Microbiology and Biotechnology, 100(13):5815-27. Read online
[3] Pereira, M. R., Mercaldi, G. F., Maester, T. C., Balan, A., Lemos, E. G. M. Est16, a New Esterase Isolated from a Metagenomic Library of a Microbial Consortium Specializing in Diesel Oil Degradation (2015). PloS One, 10(7):e0133723. Read online
[2] Garcia, R. A. M., Pereira, M. R., Maester, T. C., Lemos, E. G. M. Investigation, expression and molecular modeling of ORF2, a metagenomic lipolytic enzyme (2015). Applied Biochemistry and Biotechnology, 175(8):3875-87. Read online
[1] Pereira, M. R., Maester, T. C., Alves, L. M. C., Lemos, E. G. M. Book chapter: The use of lipolytic enzymes in biodiesel production (2012). In: Bioenergy. Development, Research and Innovation, v.1, p. 319-339.
